Encapsulation of Brunfelsia pauciflora microprogated shoots as a suitable conservation protocol

The percent work aimed to establish an efficient in vitro propagation and conservation protocol for Brunfelsia pauciflora plant using encapsulation techniques that allow the genetic stability of conserved material. effect growth regulators types (BAP, Kin, 2ip, NAA) concentrations at 0.0, 1.0, 2.0 mg/l were stud-ied during establishment phase comparison with a hormonal-free medium. In proliferated shootlets encapsulated various levels sodium alginate 1, 2, 3% (W/V) calcium chloride 2.5,5 10 %. regrowth ability explants was evaluated after 3, 5, 7 weeks compared non-encapsulated buds. findings current study proved establish-ment phase, applying BAP gave best results three successive subcultures. treatment 2ip (2.0mg/l) + NAA considered ideal shooting rooting Brunfelsia. Successful recovery from low concentra-tions (1%) 2.5% could survive high-est (100%) periods 7weeks. Increasing 5% same concentration caused gradually reduced survival 53.33% increasing conservations period (from 3 7weeks). It is first paper on application syndetic seed technique shortest renovation whose stabilization by analysis RAPD similarity ranged 56.1 80%. These confirmed anatomical sections made leaf blade.